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Alomone Labs, LTD
Dopamine Transporter (DAT) (extracellular) Polyclonal Antibody
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Dopamine Transporter (DAT) (extracellular) Antibody (AMT-003-200UL) in ICC/IF
Expression of DAT in rat PC12 cells - Cell surface detection of DAT in live intact rat pheochromocytoma PC12 cells. A. Cells stained with Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:100), followed by goat Anti-rabbit-Alexa Fluor- 594 secondary Antibody (red). B. Live view of the cells.
产品信息
AMT-003-200UL
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
(C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT, 2nd extracellular loop
偶联物
形式
Lyophilized
浓度
0.8 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DA transporter; DAT; dopamine transporter 1; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3; solute carrier family 6, member 3
基因别名: DAT; Dat1; Slc6a3
UniProt ID: (Rat) P23977, (Mouse) Q61327
Entrez Gene ID: (Rat) 24898, (Mouse) 13162
protease binding
receptor binding
dopamine transmembrane transporter activity
dopamine:sodium symporter activity
drug binding
monoamine transmembrane transporter activity
protein complex binding
dopamine binding
protein N-terminus binding
protein phosphatase 2A binding
neurotransmitter:sodium symporter activity
protein binding
symporter activity
primary active transporter
neurotransmitter transport
aging
lactation
sensory perception of smell
locomotory behavior
response to iron ion
response to organic cyclic compound
monoamine transport
dopamine transport
adenohypophysis development
response to nicotine
positive regulation of multicellular organism growth
regulation of dopamine metabolic process
response to cocaine
dopamine biosynthetic process
dopamine catabolic process
response to drug
response to ethanol
response to cAMP
prepulse inhibition
ammonium transmembrane transport
cation transmembrane transport
transport
organic cation transport
sodium ion transmembrane transport
