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Alomone Labs, LTD
Dopamine Transporter (DAT) (extracellular) Polyclonal Antibody
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Dopamine Transporter (DAT) (extracellular) Antibody (AMT-003-200UL) in ICC/IF
Expression of DAT in rat PC12 cells - Cell surface detection of DAT in live intact rat pheochromocytoma PC12 cells. A. Cells stained with Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003), (1:100), followed by goat Anti-rabbit-Alexa Fluor- 594 secondary Antibody (red). B. Live view of the cells.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
AMT-003-200UL
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
(C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT, 2nd extracellular loop
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.8 mg/mL
规格
160 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DA transporter; DAT; dopamine transporter 1; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; unnamed protein product
基因别名: DAT; Dat1
UniProt ID: (Mouse) Q61327, (Rat) P23977
Entrez Gene ID: (Mouse) 13162, (Rat) 24898
protease binding
receptor binding
neurotransmitter transporter activity
dopamine:sodium symporter activity
norepinephrine:sodium symporter activity
protein binding
monoamine transmembrane transporter activity
dopamine binding
amine binding
macromolecular complex binding
protein phosphatase 2A binding
heterocyclic compound binding
neurotransmitter uptake
neurotransmitter transport
amino acid transport
lactation
sensory perception of smell
locomotory behavior
response to xenobiotic stimulus
response to iron ion
adenohypophysis development
response to nicotine
sodium ion transmembrane transport
positive regulation of multicellular organism growth
regulation of dopamine metabolic process
response to cocaine
dopamine biosynthetic process
dopamine catabolic process
response to leptin
response to ethanol
cognition
dopamine uptake involved in synaptic transmission
response to cAMP
norepinephrine uptake
prepulse inhibition
dopamine uptake
hyaloid vascular plexus regression
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