Invitrogen

E-Cadherin Monoclonal Antibody (4A2C7)

Name: E-Cadherin Monoclonal Antibody (4A2C7)
Brand: Invitrogen
SKU: 18-0223

20个已发表图片

25 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

已发表图片 (20)

E-Cadherin Antibody in Immunocytochemistry (ICC/IF)

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E-Cadherin Antibody (18-0223) in ICC/IF

Nature communications 2020 - Fig. 7 CAF-S1 and CAF-S4 promote cancer cell invasion by TGFbeta, CXCL12 and NOTCH. a Up, Representative images of E-cadherin (red), F-actin (green) and DAPI (blue) in BC cells alone (-) or with CAF-S1 transfected with non-targeting (siCTL) or CXCL12-targeting (siCXCL12) siRNA. CAF-S4 images in Supplementary Fig. 5c . Scale bars, 20 mum; inset 10 mum. Down, BC cell density and E-cadherin staining alone (gra... View More

E-Cadherin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

E-Cadherin Antibody in Immunohistochemistry (IHC)

E-Cadherin Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image E-Cadherin Monoclonal Antibody (4A2C7)

产品信息

18-0223

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:200

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免疫细胞化学 (ICC/IF)

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亲和纯化 (AP)

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产品规格

种属反应

Human

已发表种属

C. elegans

宿主/亚型

Mouse

分类

Monoclonal

类型

Antibody

克隆号

4A2C7

抗原

Recombinant protein corresponding to the cytoplasmic domain of human E-cadherin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

purified

保存液

PBS with 1% BSA

内含物

0.1% sodium azide

保存条件

2-8°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2925243

产品详细信息

This monoclonal antibody reacts with human epithelial cadherin (E-cadherin, uvomorulin, Cell-CAM 120/80), a 120 kDa transmembrane glycoprotein. Reduction and/or loss of E-cadherin expression in carcinomas correlates positively with tumor cell invasion and metastasis. Altered expression of E-cadherin is common in the progression of cancers including breast carcinoma, lung carcinoma, gastric tumors, hepatocellular carcinoma, and prostatic tumors. It has recently been reported that E-cadherin is a useful marker for differentiating lobular lesions (negative) from ductal invasive lesions (positive) in breast cancer. Positive tissue: Non-neural epithelia. Reacts strongly with human E-cadherin and weakly with human P-cadherin.

Expected Staining Pattern: Mainly cellular membrane, some cytoplasmic

靶标信息

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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基因别名: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

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UniProt ID: (Human) P12830

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Entrez Gene ID: (Human) 999

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功能