Invitrogen

E2F6 Polyclonal Antibody

Name: E2F6 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95059

查看其他15个E2F6抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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E2F6 Antibody (PA5-95059) in WB

Western blot analysis of E2F6 in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: COLO320 whole cell lysate (40 µg), Lane 3: A549 whole cell lysate (40 µg), Lane 4: MCF-7 whole cell lysate (40 µg), Lane 5: SMMC whole cell lysate (40 µg). Samples were incubated with E2F6 polyclonal antibody (Product # PA5-95059).

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Immunogen image

Immunogen Image E2F6 Polyclonal Antibody

产品信息

PA5-95059

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence in the middle region of human E2F6 (162-177aa KDCAQQLFELTDDKEN).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2806865

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: HELA whole cell, COLO320 whole cell, A549 whole cell, MCF-7 whole cell, SMMC whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。