Invitrogen

E2F7 Polyclonal Antibody

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1 篇参考文献

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问题与答案

问题与答案

问题与答案

抗体检测数据 (1)
已发表图片 (4)

E2F7 Antibody in Western Blot (WB)

E2F7 Antibody in Western Blot (WB)

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E2F7 Antibody (PA5-68912) in WB

Western blot analysis of E2F7 on mouse SP2/0 cells. The sample was probed with a E2F7 polyclonal antibody (Product # PA5-68912) using a primary antibody dilution of 2.5 µg/mL.

E2F7 Antibody in Western Blot (WB)

E2F7 Antibody in Western Blot (WB)

E2F7 Antibody in Western Blot (WB)

E2F7 Antibody in Western Blot (WB)

E2F7 Antibody in Western Blot (WB)

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E2F7 Antibody in Western Blot (WB) — Western blot analysis of E2F7 on mouse SP2/0 cells. The sample was probed with a E2F7 polyclonal antibody (Product # PA5-68912) using a primary antibody dilution of 2.5 µg/mL.

E2F7 Antibody in Western Blot (WB) — 5 FIGURE E2F7 is a target of miR-379-5p. (a) The binding sites between miR-379-5p and E2F7 are shown. (b, c) Dual-luciferase reporter assay for the luciferase activity of wild and mutated E2F7 reporter after miR-379-5p overexpression in A549/DDP and H1299/DDP cells. (d) Quantitative reverse transcription PCR ( qRT-PCR) analysis of E2F7 mRNA expression in 27 DDP-sensitive and 28 DDP-resistant lung cancer tissues. (e) E2F7 mRNA expression was negatively correlated with miR-379-5p expression in DDP-resistant lung cancer tissues. (f) Western blot analysis of E2F7 protein levels in 27 DDP-sensitive and 28 DDP-resistant lung cancer tissues. (g) Western blot analysis of E2F7 protein levels in normal HBE, A549, H1299, A549/DDP and H1299/DDP cells. (h) The interference efficiency of miR-379-5p inhibitor or inhibitor NC in A549/DDP and H1299/DDP cells using qRT-PCR. (i) Western blot analysis of E2F7 protein levels in miR-379-5p up- or downregulated A549/DDP and H1299/DDP cells. *** p < 0.001 and **** p < 0.0001.

E2F7 Antibody in Western Blot (WB) — 6 FIGURE MiR-379-5p re-expression elevates DDP-resistant lung cancer cell sensitivity to DDP via targeting E2F7. (a-i) A549/DDP and H1299/DDP cells were transfected with miR-NC, miR-379-5p, miR-379-5p + pcDNA, or E2F7. (a) Western blot analysis of E2F7 protein levels in A549/DDP and H1299/DDP cells. (b) Cell counting kit-8 (CCK-8) assay of the IC50 values of A549/DDP and H1299/DDP cells to DDP. The proliferation (c, d), apoptosis (e), invasion (f) and migration (g) of transfected A549/DDP and H1299/DDP cells under 25 muM DDP treatment were determined using cell counting kit-8 (CCK-8) assay, 5-ethynyl -2'-deoxyuridine ( EdU), flow cytometry, transwell, and wound healing assays, respectively. (h, i) Western blot analysis of cyclin D1, MMP9, and MDR1 protein levels in A549/DDP and H1299/DDP cells under 25 muM DDP treatment. *** p < 0.001 and **** p < 0.0001.

E2F7 Antibody in Western Blot (WB) — 8 FIGURE Circ_0010235 silencing boosts tumor sensitivity to DDP in vivo. (a) A caliper was used to measure tumor volume of mice in each group. (b) Typical lung cancer tumors were present, and tumor weight of mice in each group was detected. (c) The expression of circ_0010235 and miR-379-5p in tumor tissues of mice in each group was analyzed using quantitative reverse transcription PCR (qRT-PCR). (d, e) Western blot analysis and immunohistochemistry (IHC) staining for E2F7 protein level in tumor tissues of mice in each group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

E2F7 Antibody in Western Blot (WB) — 7 FIGURE Circ_0010235/miR-379-5p axis can regulate E2F7 expression. (a, b) Western blot analysis of E2F7 expression in A549/DDP and H1299/DDP cells transfected with si-NC, si-circ_0010235, si-circ_0010235 + anti-miR-NC or si-circ_0010235 + anti-miR-379-5p. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Immunogen image

E2F7 Polyclonal Antibody

Immunogen Image E2F7 Polyclonal Antibody

产品信息

PA5-68912

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.5 µg/mL

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产品规格

种属反应

Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

synthetic peptide directed towards the N terminal region of mouse E2F7

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 93%; Mouse: 100%; Rabbit: 93%; Rat: 93%

靶标信息

E2F transcription factors, such as E2F7, play an essential role in the regulation of cell cycle progression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: E2F-7; FLJ12981; no; Transcription factor E2F7

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基因别名: A630014C11Rik; D10Ertd739e; E2f7

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UniProt ID: (Mouse) Q6S7F2

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Entrez Gene ID: (Mouse) 52679

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RNA polymerase II regulatory region sequence-specific DNA binding core promoter binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding identical protein binding RNA metabolism protein nucleic acid metabolism protein general transcription factor

negative regulation of transcription from RNA polymerase II promoter placenta development sprouting angiogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cellular response to DNA damage stimulus cell cycle negative regulation of cell proliferation DNA damage response, signal transduction by p53 class mediator negative regulation of cytokinesis positive regulation of DNA endoreduplication positive regulation of transcription from RNA polymerase II promoter trophoblast giant cell differentiation chorionic trophoblast cell differentiation hepatocyte differentiation negative regulation of transcription involved in G1/S transition of mitotic cell cycle negative regulation of G1/S transition of mitotic cell cycle