Invitrogen

EB1/EB2/EB3 Monoclonal Antibody (KT65)

Name: EB1\/EB2\/EB3 Monoclonal Antibody (KT65)
Brand: Invitrogen
SKU: MA5-47713

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

EB1/EB2/EB3 Antibody in Western Blot (WB)

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EB1/EB2/EB3 Antibody (MA5-47713) in WB

Western blot of EB1/EB2/EB3 in mouse brain (lanes 1-5). The blot was probed with rat EB1 monoclonal antibody (Product # MA5-47663) (lane 1), EB1/EB2/EB3 monoclonal antibody (Product # MA5-47713) (lane 2), MAPRE2 monoclonal antibody (Product # MA5-47664) (lane 3), MAPRE3 monoclonal antibody (Product # MA5-47714) (lane 4), and anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.

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产品信息

MA5-47713

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫细胞化学 (ICC/IF)

1:50

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

KT65

抗原

Clone KT65 was generated from a GST fusion protein containing amino acids from the C-terminal region mouse EB1. This sequence is highly conserved in human and rat EB1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2942694

产品详细信息

The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells. The antibody may weakly detect endogenous EB2 and EB3 in some cells and tissues.

靶标信息

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (alpha) and short (beta) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC; APC binding protein; APC-binding protein EB1; APC-binding protein EB2; EB1; EB1 protein family member 3; EB2; EB3; EBF3; End-binding protein 1; End-binding protein 2; End-binding protein 3; Microtubule RP/EB; Microtubule-associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 2; Microtubule-associated protein RP/EB family member 3; microtubule-associated protein, RP/EB family, member 1; microtubule-associated protein, RP/EB family, member 3; RP3; T-cell activation protein, EB1 family; T-cell activation protein, EB1 family, member 2

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基因别名: 5530600P05Rik; AI314113; AI462499; AI504412; AI790651; AW260097; BIM1p; C820009F03Rik; CSCSC2; D18Abb1e; D2Ertd459e; EB1; EB2; EB3; EBF3; EBF3-S; MAPRE1; MAPRE2; MAPRE3; RP1; RP3