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Invitrogen
EBP50 Polyclonal Antibody
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EBP50 Antibody (PA1-090) in ICC/IF
Immunofluorescent analysis of EBP50 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EBP50 Rabbit Polyclonal Antibody (Product # PA1-090) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
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.jpg?time=20240507?time=20240507 "EBP50 Antibody (PA1-090) in IHC (P)")
产品信息
PA1-090
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Dog,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Bacterially expressed, His-tagged human EBP50.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.
PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium.
The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50.
靶标信息
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
基因别名: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1
UniProt ID: (Human) O14745, (Mouse) P70441
Entrez Gene ID: (Human) 9368, (Mouse) 26941
receptor binding
protein binding
beta-catenin binding
chloride channel regulator activity
phosphatase binding
PDZ domain binding
beta-2 adrenergic receptor binding
protein complex binding
protein complex scaffold
protein self-association
dopamine receptor binding
growth factor receptor binding
protein domain specific binding
renal sodium ion transport
protein complex assembly
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
regulation of cell shape
regulation of cell size
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of phosphatidylinositol 3-kinase signaling
Wnt signaling pathway
cAMP-mediated signaling
gland morphogenesis
microvillus assembly
actin cytoskeleton organization
negative regulation of cell migration
cellular phosphate ion homeostasis
regulation of sodium:proton antiporter activity
negative regulation of sodium:proton antiporter activity
bile acid secretion
glutathione transport
negative regulation of catenin import into nucleus
establishment of nucleus localization
regulation of excretion
establishment of epithelial cell apical/basal polarity
regulation of protein kinase activity
negative regulation of mitotic cell cycle
establishment of Golgi localization
negative regulation of protein kinase B signaling
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
renal absorption
negative regulation of ERK1 and ERK2 cascade
establishment of protein localization to plasma membrane
renal phosphate ion absorption
positive regulation of intrinsic apoptotic signaling pathway
negative regulation of sodium ion transport
cellular protein localization
negative regulation of cell motility
