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Invitrogen
EEA1 Polyclonal Antibody
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EEA1 Antibody (PA1-063A) in ICC/IF
Immunofluorescent analysis of Early Endosomal Antigen 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Early Endosomal Antigen 1 Rabbit Polyclonal Antibody (Product # PA1-063A) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, ... View More
产品信息
PA1-063A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Dog,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues T(1391) P S S K K P V R V C D A C F N D L Q G(1410) of human EEA1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-063A detects early endosomal antigen 1 (EEA1) from mouse and human samples. Previous lots of this antibody have been used on hamster, canine, and rat samples.
PA1-063A has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~160 kDa protein representing EEA1 from mouse brain extract. Previous lots of this antibody have been used in immunoprecipitation and immunofluorescence procedures.
The PA1-063A immunogen is a synthetic peptide corresponding to residues T(1391) P S S K K P V R V C D A C F N D L Q G(1410) of human EEA1. The PA1-063A immunizing peptide (Cat. # PEP-079) is available for use in neutralization and control experiments.
靶标信息
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2
基因别名: A430109M19Rik; B230358H09Rik; EEA1; MST105; MSTP105; ZFYVE2
UniProt ID: (Human) Q15075, (Mouse) Q8BL66
Entrez Gene ID: (Human) 8411, (Mouse) 216238
