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Invitrogen
EEA1 Recombinant Rabbit Monoclonal Antibody (JE59-34)
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EEA1 Antibody (MA562913) in ICC/IF
Immunofluorescence analysis of EEA1 was performed using T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EEA1 Recombinant Rabbit Monoclonal Antibody (JE59-34)(Product # MA562913) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilution), for 45 minutes at room temperatu... View More
产品信息
MA562913
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE59-34
抗原
Recombinant protein within Human EEA1 aa 1-100/1,411.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive Control: HeLa cell lysate, Jurkat cell lysate, JAR cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, JAR, NIH/3T3, C6.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Cytoplasm, Early endosome membrane.
Sequence Similarities: 94% Mouse/Rat.
Predicted band size: 162 kDa
靶标信息
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2
基因别名: A430109M19Rik; B230358H09Rik; EEA1; MST105; MSTP105; ZFYVE2
UniProt ID: (Human) Q15075, (Mouse) Q8BL66
Entrez Gene ID: (Human) 8411, (Rat) 314764, (Mouse) 216238
