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Agrisera
EF1A/Elongation factor 1-alpha/EF-1-alpha Polyclonal Antibody
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EF1A/Elongation factor 1-alpha/EF-1-alpha Antibody (AS11-1633) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22°C (3) or heat treated at 38°C for 2 hours prior to protein extraction (4). As positive control 10 ng of recombinant At EF 1 A (2) were separated side by side with the plant samples on 10-15% gradient SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked following transfer with 5% low fat m... View More
产品信息
AS11-1633
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Full-length, recombinant EF1A of Arabidopsis thaliana.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 25 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Oryza sativa, Triticum aestivum
靶标信息
EF1A1, also called elongation factor 1 alpha 1, is a highly abundant cytosolic GTP binding protein that delivers aminoacyl tRNAs to the ribosome during the elongation phase of protein synthesis in plants. It cycles between GTP and GDP bound states, interacting with EF1B for nucleotide exchange and ensuring translational accuracy and efficiency. Beyond translation, EF1A1 binds actin and microtubules, contributing to cytoskeleton organization, vesicle trafficking, and responses to stress. Its expression is strong in growing tissues and can change with environmental conditions, pathogen challenge, and developmental cues. EF1A1 is often used as a reference gene for normalization in expression studies due to its stable baseline, though stability can vary with treatments, so validation is recommended.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; EF1A1; EF1ALPHA1; GRAF-1EF; LENG7; PTI1
Disclaimer
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