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Invitrogen
EGF Monoclonal Antibody (1H11)
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EGF Antibody (M805) in ICC/IF
Immunofluorescent analysis of EGF (green) in HeLa cells either left untreated (left panel) or treated with 10 ng/mL EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M805) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG seconda... View More
产品信息
M805
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
1H11
抗原
Recombinant Human EGF
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The M805 anti-EGF antibody (Clone 1H11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M806B (biotinylated conjugate of Clone 3A8).
Typical dilutions for sandwich ELISA include 1-3 µgs/mL for coating and 0.125-0.5 µg/mL for detection.
靶标信息
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor
基因别名: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
calcium ion binding
protein binding
growth factor activity
Wnt-protein binding
transmembrane receptor protein tyrosine kinase activator activity
Wnt-activated receptor activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
transmembrane signal receptor
MAPK cascade
activation of MAPKK activity
activation of MAPK activity
angiogenesis
platelet degranulation
DNA replication
signal transduction
activation of transmembrane receptor protein tyrosine kinase activity
epidermal growth factor receptor signaling pathway
STAT protein import into nucleus
positive regulation of cell proliferation
positive regulation of peptidyl-threonine phosphorylation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
positive regulation of cerebellar granule cell precursor proliferation
positive regulation of catenin import into nucleus
ERBB2 signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
positive regulation of phosphorylation
positive regulation of DNA binding
positive regulation of MAP kinase activity
positive regulation of GTPase activity
Wnt signaling pathway involved in dorsal/ventral axis specification
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
branching morphogenesis of an epithelial tube
negative regulation of secretion
canonical Wnt signaling pathway
mammary gland alveolus development
ERK1 and ERK2 cascade
regulation of calcium ion import
negative regulation of cholesterol efflux
positive regulation of hyaluronan biosynthetic process
regulation of protein localization to cell surface
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of cell motility
