Invitrogen

EGFR Monoclonal Antibody (H11)

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说明书
实验方案
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EGFR Monoclonal Antibody (H11)

产品信息

MA1-12693

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.5-1 µg/mL
查看27篇已发表文章 27篇已发表文章

免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

2-4 µg/mL
-

免疫细胞化学 (ICC/IF)

5 µg/mL
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流式细胞分析 (Flow)

-
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酶联免疫吸附实验 (ELISA)

-
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免疫沉淀 (IP)

2 µg/mg lysate
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中和实验 (Neu)

-
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凝胶阻滞分析 (GS)

-
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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

H11

抗原

HC2 20 d2 cells.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1074165

产品详细信息

MA1-12693 detects Epidermal Growth Factor Receptor in human samples.

MA1-12693 has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures. Staining of formalin-fixed tissues requires digestion with protease xxv at 1 mg/mL in PBS for 5 minutes at 37°C. Positive controls include A431 cells or placenta or squamous cell carcinoma. Suitable for Western blotting of extracellular domain of EGFR.

The MA1-12693 immunogen is HC2 20 d2 cells.

靶标信息

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone; waved 2

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基因别名: 9030024J15Rik; AI552599; EGFR; ERBB; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5

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UniProt ID: (Human) P00533, (Mouse) Q01279

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Entrez Gene ID: (Human) 1956, (Mouse) 13649

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功能
chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding glycoprotein binding protein kinase activity signal transducer activity receptor binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding epidermal growth factor binding transmembrane signal receptor
参与通路
MAPK cascade activation of MAPKK activity cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development response to stress signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway activation of phospholipase C activity salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation cerebral cortex cell migration positive regulation of cell growth positive regulation of cell migration positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway negative regulation of protein catabolic process positive regulation of phosphorylation activation of phospholipase A2 activity by calcium-mediated signaling negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of nitric oxide biosynthetic process positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation regulation of nitric-oxide synthase activity protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of cell motility translation protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development epidermis development magnesium ion homeostasis phosphorylation positive regulation of superoxide anion generation intracellular signal transduction regulation of cell proliferation ovulation cycle positive regulation of vasoconstriction positive regulation of vasodilation negative regulation of mitotic cell cycle astrocyte activation positive regulation of smooth muscle cell proliferation neuron projection morphogenesis positive regulation of inflammatory response response to calcium ion positive regulation of synaptic transmission, glutamatergic
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