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Invitrogen
EGLN1/EGLN2 Polyclonal Antibody
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EGLN1/EGLN2 Antibody (PA5-109677) in WB
Western blot analysis of EGLN1/EGLN2 in extracts of various cell lines. Samples were incubated with EGLN1/EGLN2 Polyclonal antibody (Product # PA5-109677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-109677
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.81 mg/mL
规格
81 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: DIRGDKITWI EGKEPGCETI GLLMSSMDDL IRHCNGKLGS YKINGRTKAM VACYPGNGTG YVRHVDNPNG DGRCVTCIYY LNKDWDAKVS GGILRIFPEG K
靶标信息
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Egl nine homolog 1; Egl nine homolog 2; EIT-6; Estrogen-induced tag 6; HIF-PH1; HIF-PH2; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; Hypoxia-inducible factor prolyl hydroxylase 2; PHD1; PHD2; Prolyl hydroxylase domain-containing protein 1; Prolyl hydroxylase domain-containing protein 2; Prolyl hydroxylase EGLN2; SM-20
基因别名: C1orf12; EGLN1; EGLN2; EIT6; PNAS-118; PNAS-137
Entrez Gene ID: (Human) 54583, (Human) 112398
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