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EP300 Monoclonal Antibody (1D2)

查看其他16个p300抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (4)

EP300 Antibody in Immunocytochemistry (ICC/IF)

EP300 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

EP300 Antibody (H00002033-M02) in ICC/IF

Immunofluorescence of monoclonal antibody to EP300 on HeLa cell. Antibody concentration is 10 µg/mL.

EP300 Antibody in Immunocytochemistry (ICC/IF)

EP300 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EP300 Antibody in Western Blot (WB)

EP300 Antibody in ELISA (ELISA)

Product Image Gallery

EP300 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to EP300 on HeLa cell. Antibody concentration is 10 µg/mL.

EP300 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunoperoxidase of monoclonal antibody to EP300 on formalin-fixed paraffin-embedded human colon. Antibody concentration is 3 µg/mL.

EP300 Antibody in Western Blot (WB) — EP300 monoclonal antibody (M02), clone 1D2 Western Blot analysis of EP300 expression in HeLa S3 NE (Cat # L013V3).

EP300 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged EP300 is approximately 0.03 ng/mL as a capture antibody.

Immunogen image

EP300 Monoclonal Antibody (1D2)

Immunogen Image EP300 Monoclonal Antibody (1D2)

产品信息

H00002033-M02

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

-

免疫组化（石蜡） (IHC (P))

3 µg/mL

-

免疫细胞化学 (ICC/IF)

10 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.03 ng/mL

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1D2

抗原

EP300 (NP_001420, 731 a.a. approximately 830 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

50 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Sequence of this protein is as follows: PPLQHHGQLA QPGALNPPMG YGPRMQQPSN QGQFLPQTQF PSQGMNVTNI PLAPSSGQAP VSQAQMSSSS CPVNSPIMPP GSQGSHIHCP QLPQPALHQN

靶标信息

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein isonicotinyltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300

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基因别名: EP300; KAT3B; MKHK2; P300; RSTS2

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UniProt ID: (Human) Q09472

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Entrez Gene ID: (Human) 2033

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transcription cofactor binding transcription coactivator binding p53 binding DNA binding chromatin binding damaged DNA binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding zinc ion binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity acetyltransferase activity transferase activity transferase activity, transferring acyl groups ligand-dependent nuclear receptor binding chromatin DNA binding histone acetyltransferase activity (H3-K18 specific) histone H2B acetyltransferase activity histone acetyltransferase activity (H3-K27 specific) metal ion binding tau protein binding androgen receptor binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding peptide-lysine-N-acetyltransferase activity protein propionyltransferase activity pre-mRNA intronic binding STAT family protein binding peptide 2-hydroxyisobutyryltransferase activity peptide lactyltransferase (CoA-dependent) activity histone lactyltransferase (CoA-dependent) activity acetylation-dependent protein binding peptide crotonyltransferase activity peptide butyryltransferase activity histone crotonyltransferase activity histone butyryltransferase activity DNA-binding transcription factor binding histone reader activity histone H3K122 acetyltransferase activity

autophagosome assembly negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis thigmotaxis behavioral defense response stimulatory C-type lectin receptor signaling pathway gluconeogenesis glycolytic process regulation of glycolytic process chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade nervous system development heart development skeletal muscle tissue development learning or memory circadian rhythm lipid biosynthetic process organ morphogenesis regulation of autophagy negative regulation of autophagy positive regulation of gene expression regulation of mitochondrion organization positive regulation of neuron projection development N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of protein oligomerization cellular response to UV multicellular organism growth megakaryocyte development endodermal cell differentiation swimming TORC1 signaling TORC2 signaling positive regulation of protein import into nucleus intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator protein kinase B signaling response to estrogen positive regulation by host of viral transcription fat cell differentiation negative regulation of gluconeogenesis positive regulation of gene expression, epigenetic positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade rhythmic process protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling canonical Wnt signaling pathway face morphogenesis regulation of androgen receptor signaling pathway peptidyl-lysine propionylation protein localization to chromatin cellular response to leucine tricarboxylic acid metabolic process T-helper 17 cell lineage commitment regulation of tubulin deacetylation peptidyl-lysine crotonylation peptidyl-lysine butyrylation regulation of cellular response to heat positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of T-helper 17 cell lineage commitment