Search
Agrisera
EPSP synthase/3-phosphoshikimate 1-carboxyvinyltransferase (chloroplastic) Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 2
EPSP synthase/3-phosphoshikimate 1-carboxyvinyltransferase (chloroplastic) Antibody (AS14-2782) in WB
0.5 µg of purified Arabidopsis thaliana EPSPS was separated on 10% SDS-PAGE and blotted 2 h to nitrocellulose Hibond ECLmembrane (GE Healthcare) using semi-dry or tank transfer. Blots were blocked with for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody in TBS-T with (0.1% Tween 20) at a dilution of 1:1,000 for 1 h at RT with agitation. The antibody solution was decan... View More
产品信息
AS14-2782
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana EPSP synthase.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
5-enolpyruvylshikimate-3-phosphate (EPSP) synthase is a conserved, soluble enzyme of the shikimate pathway found in bacteria, fungi, algae and plants but absent from mammals; in bacteria it functions in the cytosol, while in plants it is encoded in the nucleus and typically imported into plastids via an N-terminal targeting peptide. The protein is generally a monomeric enzyme of the enolpyruvyl transferase family that adopts a two-domain architecture connected by flexible hinge segments; ligand binding drives a large open-to-closed conformational change that brings the domains together to form the active site cleft. Catalytically, EPSP synthase (EC 2.5.1.19) performs an ordered bi-substrate transferase reaction in which shikimate-3-phosphate binds first, followed by phosphoenolpyruvate, to generate 5-enolpyruvylshikimate-3-phosphate and inorganic phosphate, thereby supporting chorismate formation and downstream biosynthesis of the aromatic amino acids phenylalanine, tyrosine and tryptophan and multiple chorismate-derived metabolites. Because glyphosate competes with phosphoenolpyruvate by binding the second-substrate site on the enzyme-shikimate-3-phosphate complex, EPSP synthase is a major herbicide target; naturally occurring sequence classes with reduced glyphosate sensitivity have been described in some microbes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; aroA; EC 2.5.1.19; EPSPS
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
