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Invitrogen
ERCC1 Polyclonal Antibody
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FIGURE: 1 / 1
ERCC1 Antibody (PA5-92374) in WB
Western blot analysis of ERCC1 in extracts of rat skeletal muscle. Samples were incubated with ERCC1 Polyclonal antibody (Product # PA5-92374) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-92374
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_9737301)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.95 mg/mL
Amount
95 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Raji, MCF-7, PC-3, HepG2, HeLa, Mouse liver, Mouse brain; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: MDPGKDKEGV PQPSGPPARK KFVIPLDEDE VPPGVAKPLF RSTQSLPTVD TSAQAAPQTY AEYAISQPLE GAGATCPTGS EPLAGETPNQ ALKPGAKSNS IIVSPRQRGN PVLKFVRNVP WEFGDVIPDY VLGQSTCALF LSLRYHNLHP DYIHGRLQSL GKNFALRVLL VQVDVKDPQQ ALKELAKMCI LADCTLILAW SPEEAGRYLE TYKAYEQKPA DLLMEKLEQD FVSRVTECLT TVKSVNKTDS QTLLTTFGSL EQLIAASRED LALCPGLGPQ KVRALGKNPR SWGKERAPNK HNLRPQSFKV KKEPKTRHSG FRL
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. It is responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). It participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: clone 2B3; DNA excision repair protein ERCC-1; excision repair cross-complementation group 1; excision repair cross-complementing 1; excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); unnamed protein product
Gene Aliases: COFS4; Ercc-1; ERCC1; RAD10; UV20
UniProt ID: (Mouse) P07903
Entrez Gene ID: (Human) 2067, (Rat) 292673, (Mouse) 13870
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
DNA binding
damaged DNA binding
single-stranded DNA binding
protein binding
3' overhang single-stranded DNA endodeoxyribonuclease activity
promoter-specific chromatin binding
molecular_function
protein C-terminus binding
TBP-class protein binding
protein domain specific binding
structure-specific DNA binding
nuclease activity
endonuclease activity
hydrolase activity
endodeoxyribonuclease
telomere maintenance
DNA repair
nucleotide-excision repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
mitotic recombination
response to oxidative stress
regulation of telomere maintenance
negative regulation of telomere maintenance
telomeric DNA-containing double minutes formation
UV-damage excision repair
t-circle formation
positive regulation of t-circle formation
negative regulation of protection from non-homologous end joining at telomere
pyrimidine dimer repair by nucleotide-excision repair
DNA catabolic process, endonucleolytic
replicative cell aging
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
syncytium formation
cellular response to DNA damage stimulus
germ cell development
spermatogenesis
response to nutrient
cell proliferation
male gonad development
UV protection
response to sucrose
response to X-ray
multicellular organism aging
post-embryonic hemopoiesis
multicellular organism growth
interstrand cross-link repair
isotype switching
cell development
oogenesis
embryonic organ development
chromosome organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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