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Agrisera
ERD7/Early Response to Dehydration 7 Polyclonal Antibody
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ERD7/Early Response to Dehydration 7 Antibody (AS19-4317) in WB
Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf with protein extraction buffer (Tris-HCl 50 m M p H 8.0,150 m M Na Cl, 1% Triton X-100,0.1% SDS, 0.5% Na-Deoxycholate, 2 m M PMSF, 2 m M DTT) and denatured with LaemmLi sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blot ... View More
产品信息
AS19-4317
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana ERD7.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
ERD7 encodes a stress-responsive senescence/dehydration-associated protein in Arabidopsis thaliana that is predominantly associated with cellular membranes, with evidence supporting localization to the plasma membrane and sequence-based inference also suggesting nuclear localization. The gene produces a protein with a conserved senescence/spartin-associated domain (pfam06911; InterPro IPR009686) in its C-terminal region and is encoded by a multi-exon locus, consistent with regulated expression and transcript complexity typical of stress-inducible plant genes. Functionally, ERD7 is induced by dehydration and other abiotic stresses (including cold, salt, and high light) and contributes to adaptive remodeling of membrane properties: ERD7 family loss alters negatively charged phospholipid interactions, perturbs cold-induced phosphoinositide dynamics (including PIP2), and reduces membrane fluidity, supporting a role in maintaining membrane lipid composition and function during cold stress and development. In addition, ERD7 acts as a positive regulator of leaf senescence, where phosphorylation at Thr-225 and Ser-262 promotes age-dependent and stress-induced senescence at least in part by modulating reactive oxygen species accumulation and upregulating NADPH oxidase-encoding genes involved in ROS production.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G17840; T13L16.14
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