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EZH1 Polyclonal Antibody
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EZH1 Antibody (600-401-GT9) in WB
Western Blot of Rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: NIH/3T3 WCL p/n W10-000-358. Lane 2: NIH/3T3 nuclear extract p/n W10-001-A74. Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in NIH/3T3 nuclear extract p/n W10-001-A74.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
600-401-GT9
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-EZH1 is directed against human EZH1 at an internal position. A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.
靶标信息
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Enhancer of zeste homolog 1; ENX-2; Histone-lysine N-methyltransferase EZH1
基因别名: ENX-2; Enx2; EZH1; KIAA0388
UniProt ID: (Mouse) P70351
Entrez Gene ID: (Mouse) 14055
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