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Invitrogen
Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), eBioscience™
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Endomucin Antibody (14-5851-82) in WB
Western blot was performed using Anti-Endomucin Monoclonal Antibody (Product # 14-5851-85) and an 80kDa band corresponding to Endomucin was observed across tissue lysates tested except Mouse Bone and Mouse Adipose. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Mouse Lung (Lane 2), Mouse Bone (Lane 3) and Mouse Adipose (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting Syst... View More
产品信息
14-5851-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioV.7C7 (V.7C7)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, which was identified in a search for cell-surface expressed endothelial cell markers. Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however, an exception is the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.
Applications Reported: This eBioV.7C7 (V.7C7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin embedded tissue sections.
Applications Tested: This eBioV.7C7 (V.7C7) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Endomucin (EMCN) also known as endothelial sialomucin or mucin-like sialoglycoprotein interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Endomucin; endomucin-1; Endomucin-1/2; MUC-14; mucin 14, endothelial; Mucin-14
基因别名: 0610012K22Rik; AI315669; Emcn; Muc14
UniProt ID: (Mouse) Q9R0H2
Entrez Gene ID: (Mouse) 59308
