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Invitrogen
Endostatin Polyclonal Antibody
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FIGURE: 1 / 10
Endostatin Antibody (PA1-601) in ICC/IF
Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) ... View More
Product Details
PA1-601
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides corresponding to residues R(1718) R L M E S Y C E T W R T E(1731) of Mouse Endostatin and R(1699) R L T E S Y C E T W R T E(1712) of Human Endostatin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-601 detects recombinant human and mouse endostatin.
PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin.
PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin.
PA1-601 can be used with blocking peptide PEP-112.
Target Information
Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antiangiogenic agent; Collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; endostatin; FLJ27325; FLJ34914; MGC74745; multi-functional protein MFP; procollagen, type XVIII, alpha 1
Gene Aliases: COL18A1; GLCC; KNO; KNO1; KS
UniProt ID: (Human) P39060, (Mouse) P39061
Entrez Gene ID: (Human) 80781, (Mouse) 12822
skeletal system development
angiogenesis
endothelial cell morphogenesis
cell adhesion
visual perception
negative regulation of cell proliferation
response to xenobiotic stimulus
anatomical structure morphogenesis
organ morphogenesis
regulation of cell proliferation
response to hydrostatic pressure
positive regulation of cell proliferation
extracellular matrix organization
positive regulation of cell migration
positive regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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