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MA3-005 detects ET-1 from human, sheep, mouse and rat tissues. This antibody shows little cross-reactivity to related peptides and no known cross-reactivity to non-related peptides.
MA3-005 has been successfully used in Western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and radioimmune assay procedures. Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with MA3-005 results in staining of endothelial cells. Radioimmune assays using MA3-005 can be used to concentrate ET-1 in solution (e.g. serµm/plasma, milk, urine). This antibody is not recommended for human placenta tissue or HepG2 cells in Western blot applications.
For immunohistochemistry with MA3-005 formalin-fixed, paraffin-embedded tissue sections has been used.
The MA3-005 antigen is purified human endothelin-1. Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.
The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Big endothelin 1; Big ET; Big ET 1; Big ET1; BigET; Endothelin-1; Et; ET-1; ppET-1; PPET1; preproendothelin; Preproendothelin-1
基因别名: ARCND3; EDN1; ET-1; ET1; HDLCQ7; PPET-1; PPET1; preproET; QME
UniProt ID: (Sheep) Q9BG76, (Human) P05305, (Rat) P22388, (Mouse) P22387
Entrez Gene ID: (Sheep) 443498, (Human) 1906, (Rat) 24323, (Mouse) 13614