Invitrogen

Enkurin Polyclonal Antibody

查看其他11个Enkurin抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

Enkurin Antibody in Immunocytochemistry (ICC/IF)

Enkurin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Enkurin Antibody (PA5-100999) in ICC/IF

Immunofluorescent analysis of Enkurin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Enkurin polyclonal antibody (Product # PA5-100999) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Enkurin Antibody in Immunocytochemistry (ICC/IF)

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Enkurin Antibody in Western Blot (WB)

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Enkurin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Enkurin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Enkurin polyclonal antibody (Product # PA5-100999) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Enkurin in mouse liver tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Enkurin polyclonal antibody (Product # PA5-100999) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Enkurin in mouse heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Enkurin polyclonal antibody (Product # PA5-100999) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Enkurin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Enkurin in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Enkurin polyclonal antibody (Product # PA5-100999) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Enkurin Antibody in Western Blot (WB) — Western blot analysis of Enkurin in 293 cell lysate. Samples were incubated with Enkurin polyclonal antibody (Product # PA5-100999).

Immunogen image

Enkurin Polyclonal Antibody

Immunogen Image Enkurin Polyclonal Antibody

产品信息

PA5-100999

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human ENKUR(Accession Q8TC29), corresponding to amino acid residues N109-C159.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total C10orf63.

靶标信息

Enkurin interacts with transient receptor potential canonical.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Enkurin

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基因别名: 4933434I06Rik; AU016229; C10orf63; CFAP106; ENKUR

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UniProt ID: (Human) Q8TC29, (Mouse) Q6SP97

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Entrez Gene ID: (Human) 219670, (Mouse) 71233

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calmodulin binding SH3 domain binding