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Invitrogen
EphA2 Recombinant Rabbit Monoclonal Antibody (PSH09-21)
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图: 1 / 4
EphA2 Antibody (MA570076) in ICC/IF
Immunocytochemistry analysis of EphA2 in A549 cells. Samples were incubated with EphA2 monoclonal antibody (Product # MA570076) using a dilution of 1:100 in 1% BSA in PBST overnight at 4°C followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. PBS instead of the primary antibody was... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA570076
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH09-21
抗原
Recombinant protein within human EPHA2 aa 10-550.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive control: A549 cell lysate, HEK-293 cell lysate, HeLa cell lysate.
Subcellular location: Cell membrane, Cell projection, ruffle membrane, lamellipodium membrane, Cell junction, focal adhesion.
靶标信息
EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
基因别名: ECK; EPHA2
Entrez Gene ID: (Human) 1969
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