Invitrogen

EphA4 Monoclonal Antibody (4C8H5)

Name: EphA4 Monoclonal Antibody (4C8H5)
Brand: Invitrogen
SKU: 37-1600

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16个已发表图片

37 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

已发表图片 (16)
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EphA4 Antibody in Immunocytochemistry (ICC/IF)

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EphA4 Antibody (37-1600) in ICC/IF

Molecular and cellular biology 2013 - Published figure using EphA4 monoclonal antibody (Product # 37-1600) in Immunocytochemistry

EphA4 Antibody in Immunohistochemistry (IHC)

EphA4 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

EphA4 Antibody — Fig. 1 Reduction of EphA4 protein and mRNA levels in the hippocampus and cortex of APPPS1 mice. Representative images ( a ) and quantification ( b ) of a Western blot analysis of hippocampal EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot) with GAPDH protein levels as a loading control (unpaired t test and Mann-Whitney test respectively, n = 4-8 mice/group). Representative images ( c ) and quantification ( d ) of a Western blot analysis of cortical EphA4 protein levels in Ctrl and EphA4-KO mice (upper blot) and AD and AD;EphA4-KO mice (lower blot). GAPDH protein levels were assessed to control for equal loading (unpaired t test, n = 4 mice/group). e Representative images of RNA scope with specific probes for EphA4 in the DG, CA3, and CA1 regions of the hippocampus of Ctrl and EphA4-KO mice. Hoechst was used to stain cell nuclei. * p <= 0.05, ** p <= 0.01. Scale bar = 100 mum. Abbreviation: Ctrl control {KO}

Immunogen image

Immunogen Image EphA4 Monoclonal Antibody (4C8H5)

产品信息

37-1600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

3-10 µg/mL

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

5 µg

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抑制试剂 (IA)

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亲和纯化 (AP)

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产品规格

种属反应

Chicken, Human, Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

4C8H5

抗原

A synthetic peptide derived from the C-terminus human EphA4 receptor protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533301

产品详细信息

This antibody is specific for the endogenous EphA4 receptor protein. No cross-reactivity with EphA5 or EphA7 has been detected. On western blots, this antibody identifies a band at ~110-120 kDa. Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by western blot and immunoprecipitation and on transfected 293 cells and mouse hippocampus by immunohistochemistry.

靶标信息

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Cek8; EK8; epa4; EPH-like kinase 8; Ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; TYRO1 protein tyrosine kinase; Tyrosine-protein kinase receptor MPK-3; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase receptor SEK-1; Tyrosine-protein kinase TYRO1

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基因别名: 2900005C20Rik; AI385584; CEK8; EPHA4; HEK8; rb; SEK; Sek1; TYRO1

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UniProt ID: (Chicken) Q07496, (Human) P54764, (Mouse) Q03137

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Entrez Gene ID: (Chicken) 395559, (Human) 2043, (Mouse) 13838

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