Invitrogen

ErbB2 (HER-2) Monoclonal Antibody (MJD2), eBioscience™

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产品信息

14-9757-82

应用
建议稀释比
已发表文章

免疫印迹 (WB)

2 µg/mL
-

免疫组化（石蜡） (IHC (P))

5 µg/mL
-

免疫细胞化学 (ICC/IF)

5 µg/mL
-
产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MJD2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572990

产品详细信息

Description: This MJD2 monoclonal antibody reacts with HER-2, the product of the ErbB2 gene and a member of the human epidermal growth factor receptor (EGFR) family. HER-2 is a 185 kDa transmembrane receptor with an extracellular binding domain and an intracellular tyrosine kinase domain. Activation of HER-2 occurs following formation of homodimers or heterodimers with ligand-bound EGFR family members, HER3 or HER4. Overexpression of HER-2 results in auto-phosphorylation and increased proliferation and invasiveness and is associated with subsets of multiple cancer types such as breast, ovarian, cervical, uterine, and gastric. The use of HER-2 targeted agents has been used extensively to treat HER-2-positive breast cancer, but a recent study found that these agents can also block the proliferation of breast cancer stem cells found in HER-2-negative patients and suggests the importance of HER-2 as a target for multiple types of breast cancer. The MJD2 antibody recognizes the extracellular domain of HER-2 but at an epitope distinct from that recognized by the 4D5 (Herceptin) and SP3 monoclonal antibodies.

Applications Reported: This MJD2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This MJD2 antibody has been tested by immunhistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human BT474 cells and can be used at less than or equal to 5 µg/mL. This MJD2 antibody has been tested by western blot analysis of reduced human BT474 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

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基因别名: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1

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UniProt ID: (Human) P04626

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Entrez Gene ID: (Human) 2064

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功能
RNA polymerase I core binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding protein C-terminus binding growth factor binding protein phosphatase binding identical protein binding ErbB-3 class receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity protein dimerization activity transmembrane signal receptor
参与通路
MAPK cascade positive regulation of protein phosphorylation transcription, DNA-templated protein phosphorylation signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway peripheral nervous system development heart development neuromuscular junction development motor neuron axon guidance cell proliferation phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation positive regulation of cell growth regulation of microtubule-based process negative regulation of immature T cell proliferation in thymus ERBB2 signaling pathway wound healing myelination positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of translation regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase I promoter positive regulation of transcription from RNA polymerase III promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling oligodendrocyte differentiation positive regulation of epithelial cell proliferation regulation of ERK1 and ERK2 cascade cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus positive regulation of protein targeting to membrane regulation of cell motility
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