Invitrogen

Estrogen Receptor alpha Monoclonal Antibody (SP1)

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256 篇参考文献
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Estrogen Receptor alpha Monoclonal Antibody (SP1)

产品信息

MA1-39540

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:25
查看27篇已发表文章 27篇已发表文章

免疫组化 (IHC)

-
查看195篇已发表文章 195篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200
查看22篇已发表文章 22篇已发表文章

免疫细胞化学 (ICC/IF)

1:25-1:250
查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

1:200
查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀 实验 (ChIP)

5 µL/10^6 cells
-

点杂交 (DB)

-
查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

-
查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

-
查看3篇已发表文章 3篇已发表文章
产品规格

种属反应

Human

已发表种属

C. elegans, Human, Mouse, Rat, Sheep, Virus

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP1

抗原

Synthetic peptide derived from C-terminus of human estrogen receptor alpha
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.014 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10979688

产品详细信息

This antibody is predicted to react with porcine based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.

靶标信息

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DKFZp686N23123; ER; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

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基因别名: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1

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UniProt ID: (Human) P03372

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Entrez Gene ID: (Human) 2099

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding
参与通路
negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth osteoblast development chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process negative regulation of gene expression positive regulation of phospholipase C activity intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity regulation of neuron apoptotic process response to estrogen positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import decidualization positive regulation of fibroblast proliferation negative regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell development Sertoli cell proliferation uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation positive regulation of ERK1 and ERK2 cascade protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus negative regulation of triglyceride metabolic process negative regulation of neuron death negative regulation of production of miRNAs involved in gene silencing by miRNA baculum development
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