Rockland

F-Box Only Protein 9 Polyclonal Antibody

Name: F-Box Only Protein 9 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-918

查看其他5个FBXO9抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

F-Box Only Protein 9 Antibody in Western Blot (WB)

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F-Box Only Protein 9 Antibody (600-401-918) in WB

Western blot using Rocklands affinity purified anti-FBOX9 antibody shows detection of a band at ~100 kDa (arrowhead) believed to correspond to FBOX9 present in a MCF7 whole cell lysate (lane 1). Specific band reactivity is greatly diminished when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After bloc... View More

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Immunogen image

Immunogen Image F-Box Only Protein 9 Polyclonal Antibody

产品信息

600-401-918

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-447 of human FBOX9 protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.53 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human FBOX9 protein. A BLAST analysis was used to suggest cross reactivity with FBOX9 protein from human, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with homologues from zebrafish, bovine and mouse (94% homology) as well as chicken (88%) and Xenopus laevis (82%). Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends. Reactivity against homologues from other sources is not known.

靶标信息

FBXO9 (F-box protein 9) is a Ubl conjugation pathway protein and it contains one F-box domain and one TPR repeat. FBXO9 may act as a potential sepsis biomarker or therapeutic target. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which functions in phosphorylation-dependent ubiquitination.

仅用于科研。不用于诊断过程。未经明确授权不得转售。