Invitrogen

FAM50A Recombinant Rabbit Monoclonal Antibody (JE65-21)

查看其他8个FAM50A抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (12)

FAM50A Antibody in Immunocytochemistry (ICC/IF)

FAM50A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

FAM50A Antibody (MA5-44728) in ICC/IF

Immunocytochemistry analysis of FAM50A in NCI-H441 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelle... View More

FAM50A Antibody in Immunocytochemistry (ICC/IF)

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM50A Antibody in Western Blot (WB)

FAM50A Antibody in Western Blot (WB)

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FAM50A Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of FAM50A in NCI-H441 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:400 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human colon carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human pancreas tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human thyroid tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human spleen tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human skin tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded rat rectum tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:400 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FAM50A in paraffin-embedded human lung tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:200 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

FAM50A Antibody in Western Blot (WB) — Western blot analysis of FAM50A in different lysates (10 µg/Lane). Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Lane 1: RAW264.7 cell lysate; Lane 2: NIH/3T3 cell lysate. Predicted band size: 40 kDa. Observed band size: 40 kDa.

FAM50A Antibody in Western Blot (WB) — Western blot analysis of FAM50A in different lysates (10 µg/Lane). Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in FAM50A Monoclonal antibody (Product # MA5-44728) using a dilution of 1:1,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Lane 1: U87MG cell lysate; Lane 2: U937 cell lysate. Predicted band size: 40 kDa. Observed band size: 40 kDa.

Immunogen image

FAM50A Recombinant Rabbit Monoclonal Antibody (JE65-21)

Immunogen Image FAM50A Recombinant Rabbit Monoclonal Antibody (JE65-21)

产品信息

MA5-44728

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:400

-

免疫细胞化学 (ICC/IF)

1:50

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE65-21

抗原

Synthetic peptide within human FAM50A aa 1-50/339.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

May be a DNA-binding protein or transcriptional factor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: family with sequence similarity 50, member A; Protein FAM50A; Protein HXC-26; Protein XAP-5; XX-FW81657B9.2

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基因别名: 9F; D0HXS9928E; DXS9928E; FAM50A; HXC-26; HXC26; RGD1560964; XAP-5; XAP5

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UniProt ID: (Human) Q14320, (Mouse) Q9WV03

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Entrez Gene ID: (Human) 9130, (Rat) 293862, (Mouse) 108160

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poly(A) RNA binding

spermatogenesis