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Agrisera
FBA/Fructose-bisphosphate aldolase class 2 Polyclonal Antibody
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FBA/Fructose-bisphosphate aldolase class 2 Antibody (AS13-2731) in WB
From 0.15 to 5.17 µg of total protein from Synechocystis PCC 6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked with 5% milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 1 h at RT with agitation. The an... View More
产品信息
AS13-2731
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant FBA from Synechocystis.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Synechocystis PCC6803
靶标信息
FBA class 2 encodes a soluble, cytosolic class II fructose-1,6-bisphosphate aldolase that functions as a metal-dependent glycolytic/gluconeogenic enzyme. The protein is typically a ~350 aa enzyme that folds as an (alpha/beta)8 (TIM) barrel and assembles as a homodimer, with its catalytic center located at the C-terminal end of the barrel; catalysis requires a bound Zn2+ (and structural metal binding has also been described near the active site). Biochemically, class II FBA catalyzes the reversible aldol cleavage/condensation between fructose 1,6-bisphosphate and the triose phosphates dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, thereby supporting central carbon flux in glycolysis and gluconeogenesis; in some bacteria it can also contribute to alternative sugar-phosphate bypass reactions involving sedoheptulose bisphosphates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 1; 6-bisphosphate aldolase; 6-bisphosphate triosephosphate-lyase; 6-diphosphate aldolase; 6-diphosphofructose aldolase; Aldolase; Diphosphofructose aldolase; fba; fbaA; FBP aldolase; FBPA; fda; Fructoaldolase; Fructose 1; Fructose diphosphate aldolase; Fructose-1; Fructose-bisphosphate aldolase class II; Ketose 1-phosphate aldolase; Phosphofructoaldolase; Sedoheptulose bisphosphate aldolase; Zymohexase
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