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Agrisera
FBPase1/Fructose-1,6-bisphosphatase 1, chloroplastic Polyclonal Antibody
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FBPase1/Fructose-1,6-bisphosphatase 1, chloroplastic Antibody (AS19-4319) in WB
20 µg/well of total protein of Arabidopsis thaliana wildtype (1), chloroplastic FBPase mutant (2), mutant line of a different gene (3) were freshly extracted from 8-week old leaves with buffer (50 m M Tris-HCl p H 8.0,200 m M Na Cl, 10 m M DTT (dithiothreitol), 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4 X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAG... View More
产品信息
AS19-4319
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana FBPase1 (chloroplastic).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
CFBP1 encodes a nuclear gene whose product is synthesized as a chloroplastic precursor bearing an N-terminal transit peptide that targets the enzyme to the chloroplast stroma, where the mature protein functions in photosynthetic carbon metabolism. The encoded enzyme is a fructose-1,6-bisphosphatase (FBPase) that acts as a Mg2+-dependent phosphohydrolase, catalyzing dephosphorylation of fructose-1,6-bisphosphate to fructose-6-phosphate and thereby supporting Calvin-Benson cycle flux and carbohydrate production. Chloroplastic FBPase is subject to light-linked redox control, classically via thioredoxin-mediated reduction of a regulatory disulfide, coupling enzymatic activity to photosynthetic electron transport and stromal redox state. Genetic disruption of CFBP1 impairs photosynthesis and carbon partitioning, leading to reduced soluble sugars and starch accumulation and causing growth and developmental defects consistent with an essential role for the chloroplast FBPase step in sustaining plant productivity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2410002I16Rik; 5830420C16Rik; CFP1; CGBP; hCGBP; HsT2645; PCCX1; PHF18; SPP1; ZCGPC1
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