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Invitrogen
FBXO25 Polyclonal Antibody
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FBXO25 Antibody (PA5-90255) in WB
Western blot analysis of FBXO25 in extracts of various cell lines. Samples were incubated with FBXO25 Polyclonal antibody (Product # PA5-90255) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-90255
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 142-291 of human FBXO25 (NP_0363052)
偶联物
形式
Liquid
浓度
1.65 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: WQQQLQDLQM TKQVNNGLTL SDLPLHMLNN ILYRFSDGWD IITLGQVTPT LYMLSEDRQL WKKLCQYHFA EKQFCRHLIL SEKGHIEWKL MYFALQKHYP AKEQYGDTLH FCRHCSILFW KDSGHPCTAA DPDSCFTPVS PQHFIDLFKF; Positive Samples: DU145, 293T, Mouse testis; Cellular Location: Nucleus
靶标信息
FBXO25 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO25 belongs to the Fbxs class.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: F-box only protein 25; F-box protein Fbx25; MGC20256; MGC51975
基因别名: 9130015I06Rik; AI649137; FBX25; FBXO25
UniProt ID: (Human) Q8TCJ0, (Mouse) Q9D2Y6
Entrez Gene ID: (Human) 26260, (Mouse) 66822
