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Agrisera
FDX1/Ferredoxin Polyclonal Antibody
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FDX1/Ferredoxin Antibody (AS06-121) in WB
20 or 40 µg of total protein from Arabidopsis thaliana (WT) leaves or ferredoxin mutant fd 2 were separated on 15% acrylamide gel with 6 M urea. Filters were blotted on PVDF, 5 µg of soluble protein extracts from Chlamydomonas reinhardtii strain CC-4,532 were separated on 15% SDS-PAGE and blotted at 4 W for 30 min to nitrocellulose membrane (0.1 µm pore size) using semi-dry transfer. The blot was blocked with 1% non-fat dry milk overnight at room temperature (RT) with agitation. The blot was incubated in the primary antibody solution at a ... View More
产品信息
AS06-121
产品规格
种属反应
Algae,
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Native ferredoxin purified from Spinacia oleracea.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Pinus strobus, Spinacia oleracea, Synechocystis 6803 substrain PCC-M
靶标信息
Ferredoxin 1 (FDX1) encodes a nuclear-encoded mitochondrial protein that is synthesized as a precursor with an N-terminal mitochondrial targeting sequence and is imported into the mitochondrial matrix, where it functions as a small soluble electron-transfer factor. Structurally, FDX1 is a ferredoxin-type iron-sulfur protein in the mitochondrial 2Fe-2S ferredoxin family, binding a 2Fe-2S cluster via conserved cysteine residues within a ferredoxin fold that supports reversible redox cycling and transient protein-protein interactions. Biologically, FDX1 shuttles electrons from the NADPH-dependent ferredoxin reductase (FDXR) to multiple mitochondrial type I cytochrome P450 enzymes, thereby supporting key monooxygenase reactions central to steroid hormone biosynthesis and related pathways (including steps in vitamin D and bile acid metabolism). Beyond steroidogenic P450 support, experimental evidence indicates FDX1 has distinct target specificity from the paralog FDX2 and can contribute electrons to additional mitochondrial biosynthetic processes, including heme a and lipoyl cofactor biosynthesis, consistent with a broader role in mitochondrial redox chemistry and respiratory competence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: At1g10960; ATFD1; FD1; PETF2; T19D16.12; T19D16_12
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