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Agrisera
FIE/Fertilization-independent endosperm Polyclonal Antibody
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产品信息
AS12-2616
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FIE protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Recombinant FIE protein
靶标信息
FIE encodes a Polycomb group protein that localizes predominantly to the nucleus, consistent with its role in chromatin-based gene repression during Arabidopsis development. It produces a WD40 repeat-containing polypeptide that functions as a scaffold within Polycomb repressive complex 2 (PRC2), partnering with the SET domain histone methyltransferase MEDEA to regulate target gene expression. Loss-of-function alleles demonstrate that FIE is essential to prevent premature seed development: in the female gametophyte, disruption of FIE derepresses the central cell program, allowing initiation of endosperm development without fertilization and causing parent-of-origin effects on seed viability. Beyond seed formation, reduced FIE activity perturbs sporophytic development and is associated with misregulation of homeobox and other developmental regulators, highlighting a broader role for FIE-dependent PRC2 repression in maintaining correct developmental phase and organ identity programs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT3G20740; FERTILIZATION-INDEPENDENT ENDOSPERM; FERTILIZATION-INDEPENDENT ENDOSPERM 1; FIE1; FIS3
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