Invitrogen

FLI1 Polyclonal Antibody

Name: FLI1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29597
Availability: InStock

高级验证

2 篇参考文献

查看其他36个FLI1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (8)
高级验证 (2)

FLI1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

FLI1 Antibody (PA5-29597) in ICC/IF

Immunofluorescence analysis of Friend leukemia integration 1 transcription factor was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FLI1 Polyclonal Antibody (Product # PA5-29597) at (1:100 dilution) in 0.1% BSA, incubated at 4-degree Celsius overnight, and labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Anti... View More

FLI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FLI1 Antibody in Immunoprecipitation (IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

FLI1 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of Friend leukemia integration 1 transcription factor was observed in the Raji cell line, but not in K-562 while using Anti-FLI1 Polyclonal Antibody (Product # PA5-29597) in Western Blot. {RE}

产品信息

PA5-29597

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

CUT&Tag (CUT&Tag)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.22 mg/mL

122 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2547073

产品详细信息

Recommended positive controls: Jurkat, Raji, NCI-H929, BCL-1, rat kidney.

Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (83%), Chicken (91%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The FLI1 (Friend Leukemia Integration 1) gene is located on chromosome 11q24.3 and belongs to the ETS (E26 transformation-specific) family of transcription factors. Structurally, FLI1 contains an ETS domain responsible for DNA binding and a pointed domain which facilitates protein-protein interactions. Functionally, it plays a critical role in the regulation of embryonic and hematopoietic development. It is also implicated in the pathogenesis of diseases such as Ewing's sarcoma, wherein a chromosomal translocation t(11;22)(q24;q12) forms the EWS-FLI1 fusion gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。