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Invitrogen
FLI1 Polyclonal Antibody
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图: 1 / 7
FLI1 Antibody (PA5-97755) in ICC/IF
Immunofluorescence analysis of Friend leukemia integration 1 transcription factor was performed using 70% confluent log phase MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FLI1 Polyclonal Antibody (Product # PA5-97755) at (1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seconda... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-97755
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Friend leukemia integration 1 transcription factor protein (205-331AA)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
3.19 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
The FLI1 (Friend Leukemia Integration 1) gene is located on chromosome 11q24.3 and belongs to the ETS (E26 transformation-specific) family of transcription factors. Structurally, FLI1 contains an ETS domain responsible for DNA binding and a pointed domain which facilitates protein-protein interactions. Functionally, it plays a critical role in the regulation of embryonic and hematopoietic development. It is also implicated in the pathogenesis of diseases such as Ewing's sarcoma, wherein a chromosomal translocation t(11;22)(q24;q12) forms the EWS-FLI1 fusion gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alternate; ETS transcription factor; Ewing sarcoma breakpoint region 2; Friend leukemia integration 1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; similar to FLI-1; Transcription factor ERGB; unnamed protein product
基因别名: BDPLT21; EWSR2; FLI-1; FLI1; SIC-1
Entrez Gene ID: (Human) 2313
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
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