Invitrogen

FOXO6 Polyclonal Antibody

3个已发表图片

3 篇参考文献

查看其他3个FOXO6抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (4)
已发表图片 (3)

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

FOXO6 Antibody (PA5-106411) in ICC/IF

Immunofluorescent analysis of FOXO6 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and FOXO6 polyclonal antibody (Product # PA5-106411) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

FOXO6 Antibody in Immunocytochemistry (ICC/IF)

FOXO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FOXO6 Antibody in Western Blot (WB)

FOXO6 Antibody in Western Blot (WB)

FOXO6 Antibody in Western Blot (WB)

FOXO6 Antibody in Western Blot (WB)

FOXO6 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

FOXO6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of FOXO6 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and FOXO6 polyclonal antibody (Product # PA5-106411) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

FOXO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded FOXO6 in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with FOXO6 polyclonal antibody (Product # PA5-106411) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

FOXO6 Antibody in Western Blot (WB) — Western blot analysis of FOXO6 in B16F10 cell lysate (left lane: treated with blocking peptide). Samples were incubated with FOXO6 polyclonal antibody (Product # PA5-106411).

FOXO6 Antibody in Western Blot (WB) — Western blot analysis of FOXO6 in mouse brain tissue. Samples were incubated with FOXO6 polyclonal antibody (Product # PA5-106411).

FOXO6 Antibody in Western Blot (WB) — Fig. 5 AST or mGluR5 specific inhibitor MTEP reduced the levels of PKClambda-ERK1/2-FOXO6 signalling pathway proteins in the dorsal horn of the L4 ~ 5 ipsilateral and contralateral spinal cord of CFA mice. ( A - F ) Representative immunofluorescent staining of PKC lambda /ERK1/2/FOXO6 and Iba-1 in the dorsal horn of the L 4 ~ 5 ipsilateral and contralateral spinal cord of mice. Scale bar = 50 mum. ( G ) Representative bands of PKC lambda /ERK1/2/FOXO6 in the dorsal horn of the L 4 ~ 5 spinal cord of mice by western blot (WB) detection. ( H - J ) Statistical analysis of PKC lambda /ERK1/2/FOXO6 proteins in the dorsal horn of the L 4 ~ 5 spinal cord of mice. Data were presented as mean +- SD, n = 3/group. * P < 0.05, ** P < 0.01.

FOXO6 Antibody in Western Blot (WB) — Fig. 6 AST or mGluR5 specific inhibitor MTEP downregulated PKClambda-ERK1/2-FOXO6 signalling pathways in the basolateral amygdala of CFA mice. ( A - C ) Representative immunofluorescent staining of PKC lambda /ERK1/2/FOXO6 and Iba-1 in the basolateral amygdala of CFA mice. Scale bar = 50 mum. ( D ) Representative bands of PKC lambda /ERK1/2/FOXO6 in the basolateral amygdala of CFA mice. by western blot (WB) detection. ( E - G ) Statistical analysis of PKC lambda /ERK1/2/FOXO6 proteins in the basolateral amygdala of CFA mice. Data were presented as mean +- SD, n = 3/group. * P < 0.05, ** P < 0.01.

FOXO6 Antibody in Western Blot (WB) — Apelin overexpression inhibited MCs proliferation and fibrosis through the SIRT1/FOXO signaling pathway. ( A ) The acetylation status of FOXO1, FOXO2, FOXO3a, FOXO4, and FOXO6 assessed by western blotting. ( B ) The effects of SIRT1 activation on acetylation status of FOXO1, FOXO3a, and FOXO6. ( C ) COIP assay proved the interactions between SIRT1 and FOXO1, and between SIRT1 and FOXO3a. ( D ) Cell viability evaluated by CCK8 assay. ( E ) Cell proliferation determined using EDU staining. ( F ) The relative mRNA expression of PCNA and CyclinD1 detected by RT-qPCR. ( G ) The protein expression of SIRT1, alpha-SMA, fibronectin, and collagen I determined using western blotting. ( H ) The acetylation status of FOXO1 and FOXO3a assessed by western blotting. ( I ) FOXO protein nuclear translocation. ( J ) FOXO phosphorylation. * p < 0.05, ** p < 0.01, *** p < 0.001. high glucose: HG; normal glucose: NG; overexpression: OE

Immunogen image

FOXO6 Polyclonal Antibody

Immunogen Image FOXO6 Polyclonal Antibody

产品信息

PA5-106411

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human FOXO6(Accession A8MYZ6), corresponding to amino acid residues R209-A259.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total FOXO6.

靶标信息

FOXO6 is a transcriptional activator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: forkhead box O6; Forkhead box protein O6

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基因别名: Foxo6

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UniProt ID: (Mouse) Q70KY4

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Entrez Gene ID: (Rat) 313558, (Mouse) 329934

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RNA polymerase II transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding

regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated anatomical structure morphogenesis cell differentiation