Invitrogen

FUNDC1 Polyclonal Antibody

1个已发表图片

1 篇参考文献

查看其他10个FUNDC1抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)
已发表图片 (1)

FUNDC1 Antibody in Western Blot (WB)

FUNDC1 Antibody in Western Blot (WB)

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FUNDC1 Antibody (PA5-48853) in WB

Western blot analysis of FUNDC1 in Mouse brain lysate. Samples were incubated with FUNDC1 polyclonal antibody (Product # PA5-48853) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FUNDC1 Antibody in Western Blot (WB)

FUNDC1 Antibody in Western Blot (WB)

FUNDC1 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

FUNDC1 Antibody in Western Blot (WB) — Western blot analysis of FUNDC1 in Mouse brain lysate. Samples were incubated with FUNDC1 polyclonal antibody (Product # PA5-48853) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FUNDC1 Antibody in Western Blot (WB) — Western blot analysis of FUNDC1 in NCI-H460 cell line lysates. Samples were incubated with FUNDC1 polyclonal antibody (Product # PA5-48853). Lysates: 35 µg/lane. FUNDC1 protein (arrow).

FUNDC1 Antibody in Western Blot (WB) — Figure 4. EGFR inhibition enhances MARCH5-MCL1 interaction without altering MARCH5 activity. ( A ) LNCaP cells were pretreated with proteasome inhibitors MG115 (10 muM) and MG132 (10 muM) for 1 hr, followed by treatment with erlotinib (10 muM) or DMSO for 3 hr. The cell lysates were subject to immunoprecipitation using anti-MARCH5 rabbit antibody with protein A agarose, followed by immunoblotting with anti-MARCH5 rabbit antibody or anti-MCL1 mouse antibody. The decreased mobility of MCL1 in the IP lanes may reflect impaired migration due to large amounts of Ig in the sample, but phosphorylation or other posttranslational modification is possible. ( B ) SgMARCH5-KO or control LNCaP cells were pre-incubated in normal serum medium (FBS) or charcoal-stripped serum medium (CSS) for 1 day, followed by treatment with erlotinib (10 muM) for 3 hr or EGF (100 ng/ml) for 30 min. LE, long exposure. ( C ) SgMARCH5-KO LNCaP cells were treated with erlotinib (10 muM), S63845 (0.5 muM), or DMSO for 3 hr. The cell lysates were immunopurified with anti-MCL1 rabbit antibody or control rabbit IgG and protein A agarose, followed by immunoblotting with mouse antibodies targeting for indicated proteins. ( D ) SgMARCH5-KO or control LNCaP cells were treated with erlotinib (10 muM), S63845 (500 nM), or DMSO for 3 hr. SE, short exposure. ( E ) SgMARCH5-KO or control LNCaP cells were treated with erlotinib (10 muM) for 2 hr. Proteins extracted from whole cell lysates (WCL) or isolated mitochondria (Mit

Immunogen image

FUNDC1 Polyclonal Antibody

Immunogen Image FUNDC1 Polyclonal Antibody

产品信息

PA5-48853

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FUNDC1.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.35 mg/mL

140 µg

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Predicted to react with bovine, mouse and rat based on sequence homology.

靶标信息

Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: FUN14 domain-containing protein 1

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基因别名: 1500005J14Rik; 1810033P05Rik; FUNDC1

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UniProt ID: (Mouse) Q9DB70

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Entrez Gene ID: (Mouse) 72018

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molecular_function

mitophagy response to hypoxia autophagy