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Invitrogen
FXR Monoclonal Antibody (A9033A)
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FXR Antibody (417200) in IHC (F)
Immunohistochemistry analysis of FRX in frozen rat liver tissues. The sample was inclubated in FRX monoclonal antibody (Product # 417200) at a dilution of 3~20 μg/mL. Section was fixed with 4% paraformaldehyde-fixed in immersion, activated with 5% TritonX-100 for 5 minutes, and blocked with 3% H2O2 in methanol.
产品信息
417200
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
A9033A
抗原
Baculovirus-expressed recombinant human FXR (2-126 aa).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
physiological saline
内含物
0.1% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody specifically recognizes human FXR and cross reacts with mouse and rat FXR.
靶标信息
FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR plays an essential role in bile acid homeostasis through the regulation of genes involved in bile acid synthesis, conjugation and enterohepatic circulation. FXR also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. In the liver FXR activates transcription of the corepressor NR0B2 thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in BA synthesis) implicating at least in part histone demethylase KDM1A resulting in epigenomic repression, and SLC10A1/NTCP (involved in hepatic uptake of conjugated BAs). FXR activates transcription of SLC27A5/BACS and BAAT (involved in BA conjugation), ABCB11/BSEP (involved in bile salt export) by directly recruiting histone methyltransferase CARM1, and ABCC2/MRP2 (involved in secretion of conjugated BAs) and ABCB4 (involved in secretion of phosphatidylcholine in the small intestine). (From Uniprot)
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Bile acid receptor; farnesoid X activated receptor; farnesoid X nuclear receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; MGC163445; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14
基因别名: AI957360; BAR; FXR; HRR-1; HRR1; NR1H4; RIP14; Rxrip14
UniProt ID: (Human) Q96RI1, (Rat) Q62735, (Mouse) Q60641
Entrez Gene ID: (Human) 9971, (Rat) 60351, (Mouse) 20186
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
steroid hormone receptor activity
transcription coactivator activity
transcription corepressor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
thyroid hormone receptor activity
protein binding
zinc ion binding
ligand-dependent nuclear receptor binding
bile acid binding
bile acid receptor activity
peptide binding
sequence-specific DNA binding
retinoid X receptor binding
chenodeoxycholic acid binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
double-stranded DNA binding
receptor activity
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
regulation of carbohydrate metabolic process
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
bile acid metabolic process
response to glucose
intracellular receptor signaling pathway
response to lipopolysaccharide
regulation of urea metabolic process
intracellular bile acid receptor signaling pathway
steroid hormone mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
negative regulation of bile acid biosynthetic process
cellular response to organonitrogen compound
cellular response to bile acid
positive regulation of glutamate metabolic process
positive regulation of ammonia assimilation cycle
nitrogen catabolite activation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
Notch signaling pathway
transcription initiation from RNA polymerase II promoter
inflammatory response
signal transduction
regulation of low-density lipoprotein particle clearance
bile acid and bile salt transport
toll-like receptor 4 signaling pathway
histone H3-R17 methylation
cellular triglyceride homeostasis
bile acid signaling pathway
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of bile acid biosynthetic process
cellular response to fatty acid
interleukin-17 secretion
regulation of cholesterol metabolic process
negative regulation of interferon-gamma secretion
negative regulation of tumor necrosis factor secretion
