Invitrogen

FZD4 Polyclonal Antibody

Name: FZD4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-47228

1 篇参考文献

查看其他12个FZD4抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

FZD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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FZD4 Antibody (PA5-47228) in IHC (F)

Immunohistochemical analysis of FZD4 in immersion fixed frozen sections of embryonic mouse neuronal tissue (E11). Samples were incubated in FZD4 polyclonal antibody (Product # PA5-47228) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma memb... View More

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Immunogen Image FZD4 Polyclonal Antibody

产品信息

PA5-47228

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4 Phe37-Glu180

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2607400

产品详细信息

In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Frizzled-8 and less than 2% cross-reactivity with rmFrizzled-3 and rmFrizzled-7 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

FZD4 is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. Frizzled 4 is widely expressed, particularly in heart, skeletal muscle, ovary, and fetal kidney. Transcripts have also been identified in liver, kidney, pancreas, spleen, and fetal lung, and in smaller amounts in placenta, adult lung, prostate, testis, colon, and fetal brain.

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