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Invitrogen
Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™
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Fc Receptor Binding Inhibitor Antibody (14-9162-42) in Flow
Normal canine peripheral blood cells were unstained (orange histogram), stained with Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram), or were pretreated with Canine Fc Receptor Binding Inhibitor Purified and then stained with Rat IgG2b K Isotype Control FITC (purple histogram).Cells in the monocyte gate were used for analysis.
产品信息
14-9162-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog
已发表种属
Dog
分类
Polyclonal
类型
Antibody
偶联物
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The Canine Fc Receptor Binding Inhibitor can be used to inhibit the non-specific Fc receptor (FcR)-mediated binding of rat monoclonal antibodies used for flow cytometric analysis of canine tissue. FcR are expressed at varying levels in multiple cell lineages including high expression in myeloid cells, granulocytes, and B cells. The biological function of the FcR, including initiation of endocytosis, phagocytosis, and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which rat monoclonal antibodies will bind to canine FcR varies depending on the isotype of the monoclonal antibody.
eBioscience testing indicates that rat IgG2b isotype is blocked by Canine Fc Receptor Binding Inhibitor.
Applications Reported: Canine Fc Receptor Binding Inhibitor Purified has been reported for use in flow cytometric analysis.
To inhibit the non-specific binding of rat monoclonal antibodies add the canine Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing, proceed to stain with primary antibody.
Applications Tested: Canine Fc Receptor Binding Inhibitor Purified has been pre-titrated and tested by flow cytometric analysis on canine peripheral blood cells. This can be used at 5 µL (10 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
