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Invitrogen
Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor™ 488
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Fluorescein/Oregon Green Antibody (A-11090) in ICC/IF
Chromosome spreads were prepared from the cultured fibroblast cell line MRC-5 and hybridized with an a-satellite probe labeled with the Oregon Green® 488 dye and specific for chromosome 17. The probe was labeled using the ULYSIS Alexa Fluor® 488 Nucleic Acid Labeling Kit (Product # U21659) (left panel). The signal was amplified using the Alexa Fluor® 488 conjugate of rabbit anti-fluorescein/Oregon Green® an... View More
产品信息
A-11090
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical,
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Fluorescein/Oregon Green
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
0.1M potassium phosphate, pH 8
内含物
5mM sodium azide
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The Alexa Fluor 488 conjugates can be used to amplify the fluorescence signal while still allowing fluorescein-compatible optics. Excitation 495 nm/peak emission 519 nm.
It is a good practice to centrifuge the labeled antibody solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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