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G-CSF Antibody (500-M37-500UG) in WB
Western Blot: To detect Human G-CSF by Western Blot analysis, G-CSF Monoclonal Antibody (Product # 500-M37-500UG) can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
产品信息
500-M37-500UG
产品规格
种属反应
Human
宿主/亚型
Mouse
分类
Monoclonal
类型
Antibody
抗原
E.coli-derived Animal-Free Recombinant Human G-CSF
偶联物
形式
Lyophilized
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2942197
产品详细信息
AA Sequence of recombinant protein: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography.
靶标信息
G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukemia cells. G-CSF is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. G-CSF is highly conserved among species and has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors, neutrophilic granulocytes and certain carcinoma cell lines. Clinical use of G-CSF has been approved for several therapeutic applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and AIDS. Further, G-CSF has been shown to be a marker protein for different carcinomas such as bladder cancer and dysfunction of the protein has been linked to Kostmann Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: colony stimulating factor 3 (granulocyte); CSF 3; filgrastim; G-CSF; Granulocyte colony-stimulating factor; granulocyte-colony stimulating factor; gsf3; lenograstim; MGC45931; Pluripoietin; RP23-395E10.4
基因别名: C17orf33; CSF3; CSF3OS; GCSF
UniProt ID: (Human) P09919
Entrez Gene ID: (Human) 1440
immune response
multicellular organism development
positive regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
cytokine-mediated signaling pathway
positive regulation of actin filament polymerization
granulocyte differentiation
positive regulation of protein binding
positive regulation of peptidyl-serine phosphorylation
positive regulation of transcription factor import into nucleus
positive regulation of myeloid cell differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of protein kinase B signaling
cellular response to lipopolysaccharide
cellular response to cytokine stimulus
negative regulation of neuron death
positive regulation of actin cytoskeleton reorganization
