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Invitrogen
G2E3 Polyclonal Antibody
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G2E3 Antibody (PA5-89808) in WB
Western blot analysis of G2E3 in extracts of various cell lines. Samples were incubated with G2E3 Polyclonal antibody (Product # PA5-89808) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
PA5-89808
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human G2E3 (NP_0602392)
偶联物
形式
Liquid
浓度
0.6 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MNESKPGDSQ NLACVFCRKH DDCPNKYGEK KTKEKWNLTV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVNRASKLK CCVCKKNGAS IGCVAPRCKR SYHFPCGLQR ECIFQFTGNF ASFCWDHRPV QIITSNNYRE SLPCTICLEF IEPIPSYNIL RSPCCKNAWF HRDCLQVQAI; Positive Samples: U-87MG, LO2, 293T, OVCAR3, Mouse eye, Mouse brain, Mouse liver, Rat testis; Cellular Location: Cytoplasm, Nucleus, nucleolus
靶标信息
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: G2/M phase-specific E3 ubiquitin-protein ligase; G2/M phase-specific HECT-type E3 ubiquitin transferase; G2/M-phase specific E3 ubiquitin ligase; PHD finger protein 7B
基因别名: 6030408C04Rik; 9030416F18; AW046379; D930034K21Rik; G2E3; KIAA1333; mKIAA1333; PHF7B; RGD1310263
UniProt ID: (Human) Q7L622, (Mouse) Q5RJY2
Entrez Gene ID: (Human) 55632, (Rat) 299002, (Mouse) 217558
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
DNA metabolism protein
