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GABRA1 Antibody (MA5-27703) in IHC (P)
Immunohistochemistry analysis of GABRA1 in paraffin-embedded mouse back skin. Samples were incubated with GABRA1 monoclonal antibody (Product # MA5-27703) using a dilution of 1:100 (1 hour at RT) and followed by FITC Goat Anti-Rabbit (green) at a dilution of 1:50 (1 hour at RT).
产品信息
MA5-27703
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
N95/35
抗原
Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735190
产品详细信息
1 µg/mL of MA5-27703 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.
靶标信息
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GABA(A) receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABAA alpha 1; GABAAR alpha1; GABAAR subunit alpha-1; GABRA 1; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid A receptor, alpha 1; Gamma-aminobutyric acid receptor subunit alpha-1
基因别名: ECA4; EIEE19; EJM; EJM5; Gabra-1; GABRA1
UniProt ID: (Human) P14867, (Rat) P62813, (Mouse) P62812
Entrez Gene ID: (Human) 2554, (Rat) 29705, (Mouse) 14394
GABA-A receptor activity
drug binding
GABA receptor activity
GABA-gated chloride ion channel activity
inhibitory extracellular ligand-gated ion channel activity
chloride channel activity
protein binding
benzodiazepine receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
ligand-gated ion channel
