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Agrisera
GAI/DELLA protein GAI Polyclonal Antibody
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GAI/DELLA protein GAI Antibody (AS11-1631) in WB
Samples: 1- 50 µg/well of Arabidopsis thaliana, ecotype Lens-A whole 6-week-old plant extract. 2- 50 µg/well of Pentuple mutant of all five DELLA genes (RGA, GAI, RGL 1, RGL 2 and RGL 3) generated by genetic cross; (CS 16298) 6-week-old plant extract. 3- 50 µg/well of Arabidopsis thaliana, ecotype Lens-A whole 3-week-old seedling extract. 4- 50 µg/well of Pentuple mutant of all five DELLA genes (RGA, GAI, R... View More
产品信息
AS11-1631
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
GAI encodes a nuclear DELLA-class GRAS regulatory protein that functions as a central repressor within gibberellin (GA) signaling. The polypeptide is organized with an amino-terminal regulatory region containing the conserved DELLA and adjacent VHYNP and LExLE motifs that mediate GA-dependent binding to the soluble GA receptor GID1, and a carboxy-terminal GRAS domain typical of plant transcriptional regulators that supports protein-protein interactions and transcriptional control. In the presence of bioactive GA, GID1 engages the GAI DELLA region to form a hormone-dependent complex that promotes relief of DELLA-mediated growth restraint, commonly through destabilization and turnover via the ubiquitin-26S proteasome pathway; conversely, GA-insensitive variants with lesions in the DELLA region fail to interact productively with GID1 and remain active repressors. Through this receptor-coupled "derepression" mechanism, GAI limits GA-responsive gene expression and restrains cell proliferation/expansion, thereby modulating major developmental outputs such as stem elongation, vegetative growth, and timing of floral initiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G14920; F10B6.34; F10B6_34; GAI PROTEIN; GIBBERELLIC ACID INSENSITIVE; RESTORATION ON GROWTH ON AMMONIA 2; RGA2
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