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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GATA3 Antibodies
          This clone is available in multiple conjugated formats in both full and sample sizes. For more options, search using the clone ID "TWAJ" or explore the "Additional formats" section on this page.

          Invitrogen

          GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ), Alexa Fluor™ Plus 647

          高级验证
          查看其他83个GATA3抗体

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          说明书
          实验方案
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          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ), Alexa Fluor™ Plus 647

          • 抗体检测数据 (4)
          • 高级验证 (2)
          GATA-3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GATA-3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 6

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          GATA-3 Antibody (740023TP647) in ICC/IF

          Immunofluorescent analysis of GATA-3 was performed using 70-80% confluent MCF-7 and HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ), Alexa Fluor™ Plus 647 (Product # 740023TP647, 1:100). Panel a) shows representative images of cells that were stained for detection and localization of GATA-3 (red). Panel b) shows representative ima... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          GATA-3 Antibody in Immunocytochemistry (ICC/IF)
          GATA-3 Antibody in Immunocytochemistry (ICC/IF)
          GATA-3 Antibody in Flow Cytometry (Flow)
          GATA-3 Antibody in Flow Cytometry (Flow)
          GATA-3 Antibody
          GATA-3 Antibody

          产品信息

          740023TP647

          应用
          建议稀释比
          已发表文章

          免疫细胞化学 (ICC/IF)

          1:50-1:200
          -

          流式细胞分析 (Flow)

          0.1-0.5 µg/test
          -
          产品规格

          种属反应

          Human

          宿主/亚型

          Rat / IgG2b, kappa

          Expression System

          Expi293

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          TWAJ

          偶联物

          Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647
          • Unconjugated
          • Alexa Fluor Plus 488 
          • Alexa Fluor Plus 555 
          • Request custom conjugation

          激发/发射光谱

          658/675 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          1.0 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          proprietary buffer, pH 6.8

          内含物

          0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

          保存条件

          4°C, store in dark, DO NOT FREEZE!

          运输条件

          Wet ice

          RRID

          AB_3666218

          产品详细信息

          Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

          Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

          Applications Tested: This TWAJ antibody has been tested by immunocytochemistry and flow cytometric analysis of MCF-7 cells. This may be used for immunocytochemistry at 2 µg/mL and for flow cytometry at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: GATA binding protein-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

          查看更多 收起

          基因别名: GATA3; HDR; HDRS

          查看更多 收起

          UniProt ID: (Human) P23771

          查看更多 收起

          Entrez Gene ID: (Human) 2625

          查看更多 收起

          功能
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding DNA-binding transcription factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate determination neuron migration type IV hypersensitivity kidney development mesonephros development lens development in camera-type eye pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling transcription from RNA polymerase II promoter defense response humoral immune response signal transduction axon guidance heart development blood coagulation negative regulation of cell proliferation male gonad development response to virus anatomical structure morphogenesis post-embryonic development organ morphogenesis positive regulation of signal transduction response to gamma radiation positive regulation of endothelial cell migration regulation of neuron projection development phosphatidylinositol 3-kinase signaling erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-4 production negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process inner ear morphogenesis response to drug regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of fat cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development digestive tract development developmental growth anatomical structure formation involved in morphogenesis negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of histone H3-K14 acetylation otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration negative regulation of DNA demethylation regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of histone H3-K9 acetylation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation
          It has to be done as per old AB suggested Products section.
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