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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GATA2/GATA3 Antibodies

          Invitrogen

          GATA2/GATA3 Polyclonal Antibody

          查看其他4个GATA2/GATA3抗体

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          Cite GATA2/GATA3 Polyclonal Antibody

          • 抗体检测数据 (2)
          GATA2/GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          GATA2/GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 2

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          GATA2/GATA3 Antibody (PA5-101005) in IHC (P)

          Immunohistochemistry analysis of paraffin-embedded GATA2/GATA3 in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with GATA2/GATA3 polyclonal antibody (Product # PA5-101005) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          GATA2/GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA2/GATA3 Antibody in Western Blot (WB)
          GATA2/GATA3 Polyclonal Antibody

          产品信息

          PA5-101005

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000-1:3,000
          -

          免疫组化(石蜡) (IHC (P))

          1:50-1:200
          -

          免疫细胞化学 (ICC/IF)

          Assay-dependent
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          A synthesized peptide derived from human GATA2/3, corresponding to a region within C-terminal amino acids.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.4, with 50% glycerol

          内含物

          0.02% sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2850483

          产品详细信息

          Antibody detects endogenous levels of total GATA2/3.

          靶标信息

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: Endothelial transcription factor GATA-2; GATA-binding factor 3; GATA-binding protein 2; GATA-binding protein 3; GATA-bindning protein 2; gata2 {ECO:0000312|EMBL:AAK51128.1}; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3

          查看更多 收起

          基因别名: DCML; Gata-2; Gata-3; GATA2; GATA3; HDR; HDRS; IMD21; jal; MONOMAC; NFE1B

          查看更多 收起

          UniProt ID: (Human) P23769, (Human) P23771, (Mouse) O09100, (Rat) Q924Y4, (Mouse) P23772

          查看更多 收起

          Entrez Gene ID: (Human) 2624, (Human) 2625, (Mouse) 14461, (Rat) 25159, (Mouse) 14462, (Rat) 85471

          查看更多 收起

          功能
          RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding zinc ion binding C2H2 zinc finger domain binding transcription regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription coactivator activity interleukin-2 receptor binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter urogenital system development cell fate determination neuron migration embryonic placenta development transcription, DNA-templated transcription from RNA polymerase II promoter phagocytosis positive regulation of cytosolic calcium ion concentration heart development blood coagulation organ morphogenesis tissue development regulation of primitive erythrocyte differentiation ventral spinal cord interneuron differentiation cell differentiation in hindbrain commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development pituitary gland development response to lipid somatic stem cell population maintenance regulation of histone acetylation eosinophil fate commitment inner ear morphogenesis mast cell degranulation negative regulation of fat cell differentiation positive regulation of erythrocyte differentiation negative regulation of macrophage differentiation positive regulation of megakaryocyte differentiation positive regulation of neuron differentiation negative regulation of Notch signaling pathway positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter cell maturation digestive tract development homeostasis of number of cells within a tissue positive regulation of phagocytosis positive regulation of phagocytosis, engulfment definitive hemopoiesis semicircular canal development negative regulation of fat cell proliferation GABAergic neuron differentiation negative regulation of neural precursor cell proliferation regulation of forebrain neuron differentiation in utero embryonic development type IV hypersensitivity kidney development mesonephros development lens development in camera-type eye pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling defense response humoral immune response signal transduction axon guidance negative regulation of cell proliferation male gonad development response to virus anatomical structure morphogenesis post-embryonic development positive regulation of signal transduction response to gamma radiation positive regulation of endothelial cell migration regulation of neuron projection development phosphatidylinositol 3-kinase signaling erythrocyte differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-4 production negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion regulation of cytokine biosynthetic process norepinephrine biosynthetic process response to drug regulation of CD4-positive, alpha-beta T cell differentiation regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated sympathetic nervous system development thymus development developmental growth anatomical structure formation involved in morphogenesis negative regulation of inflammatory response T cell receptor signaling pathway regulation of histone H3-K4 methylation positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development mesenchymal to epithelial transition mast cell differentiation ureteric bud formation regulation of histone H3-K27 methylation canonical Wnt signaling pathway involved in metanephric kidney development cellular response to interleukin-4 cellular response to tumor necrosis factor positive regulation of histone H3-K14 acetylation otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation interleukin-4 secretion interferon-gamma secretion lymphocyte migration negative regulation of DNA demethylation regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of histone H3-K9 acetylation positive regulation of interleukin-5 secretion positive regulation of interleukin-13 secretion positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation regulation of transcription, DNA-templated hemopoiesis cell differentiation neuron differentiation negative regulation of myeloid cell differentiation neuron fate commitment cell morphogenesis cell activation positive regulation of cytokine production immune system process regulation of transcription from RNA polymerase II promoter nervous system development negative regulation of gene expression T cell differentiation positive regulation of interleukin-13 production positive regulation of interleukin-5 production T cell differentiation in thymus positive regulation of cell differentiation embryonic organ development homeostasis of number of cells cellular response to cytokine stimulus renal system development
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