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Invitrogen
GCP4 Polyclonal Antibody
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FIGURE: 1 / 2
GCP4 Antibody (PA5-101601) in ICC/IF
Immunocytochemistry analysis of GCP4 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GCP4 polyclonal antibody (Product # PA5-101601) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101601
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TUBGCP4(Accession Q9UGJ1), corresponding to amino acid residues H193-L243.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TUBGCP4.
Target Information
The gamma-Tubulin complex is composed of gamma Tubulin and the gamma-Tubulin complexassociated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. gamma-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the gamma-Tubulin complex. GCP4 (gamma-Tubulin complex component 4), also known as TUBGCP4, is a ubiquitously expressed 667 amino acid member of the gamma-Tubulin complex that localizes to the metaphase spindle during mitosis. In response to proteosome inhibition, GCP4 exhibits increased accumulation at the pericentiolar material where it participates in microtubule organization and nucleation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gamma tubulin ring complex protein; gamma tubulin ring complex protein (76p gene); Gamma-ring complex protein 76 kDa; Gamma-tubulin complex component 4; gamma-tubulin complex protein 4; GCP-4; h76p; hGCP4; hGrip76; tubulin gamma complex associated protein 4; unnamed protein product
Gene Aliases: 4932441P04Rik; 76P; D2Ertd435e; GCP-4; GCP4; Grip76; MCCRP3; TUBGCP4
UniProt ID: (Human) Q9UGJ1, (Mouse) Q9D4F8
Entrez Gene ID: (Human) 27229, (Mouse) 51885
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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