Invitrogen

GDNF Polyclonal Antibody, PeproTech®

Name: GDNF Polyclonal Antibody, PeproTech\u00AE
Brand: Invitrogen
SKU: 500-P81-1MG

2 篇参考文献

查看其他20个GDNF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

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GDNF Antibody (500-P81-1MG) in WB

Western Blot: To detect Human GDNF by Western Blot analysis GDNF Polyclonal Antibody (Product # 500-P81-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Immunogen Image GDNF Polyclonal Antibody, PeproTech®

产品信息

500-P81-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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体外检测 (IV)

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产品规格

种属反应

Human

已发表种属

Human, Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human GDNF

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930780

产品详细信息

AA Sequence of recombinant protein: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDNF. Anti-Human GDNF-specific antibody was purified by affinity chromatography employing an immobilized hHuman GDNF matrix.

Sandwich ELISA: To detect Human GDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GDNF (500-P81Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GDNF.

Western Blot: To detect Human GDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

仅用于科研。不用于诊断过程。未经明确授权不得转售。