Invitrogen

GFAP Polyclonal Antibody

Name: GFAP Polyclonal Antibody
Brand: Invitrogen
SKU: OPA1-06100

5个已发表图片

15 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
已发表图片 (5)

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

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GFAP Antibody (OPA1-06100) in IHC (F)

Deep tissue imaging with non-curing, refractive index matched SlowFade™ Glass. Cryo-preserved rat brain sections (100 µm thick), stained for tubulin (red) with Mouse Anti-Beta3-Tubulin (Cat. No. MA1-118) and GFAP (yellow) Rabbit Anti-GFAP (Cat. No. OPA1-06100). Targets were detected with Alexa Fluor™ Plus 594 Goat Anti-Mouse (Cat. No. A-11032) and Alexa Fluor™ Plus 647 Goat Anti-Rabbit (Cat. No. A-32733). N... View More

GFAP Antibody in Immunohistochemistry (IHC)

GFAP Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Immunogen image

Immunogen Image GFAP Polyclonal Antibody

产品信息

OPA1-06100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化 (IHC)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

1:100-1:200

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免疫组化（冰冻） (IHC (F))

1:100-1:200

免疫细胞化学 (ICC/IF)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat, Zebrafish

已发表种属

C. elegans, Mouse, Rat, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human spinal cord.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_325657

产品详细信息

OPA1-06100 detects glial fibrillary acid protein (GFAP) from human, mouse, zebrafish, and rat tissues.

OPA1-06100 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects a single protein representing GFAP rat brain homogenate. Immunohistochemical staining of GFAP in rat brain with OPA1-6100 results in intense staining of astrocytes and Schwann cells.

The OPA1-06100 immunogen is cytoskeletal preparation of human spinal cord.

Store at 4°C or in small aliquots at -20°C.

靶标信息

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; zGFAP; intermediate filament protein; zrf-1 antigen

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基因别名: AI836096; ALXDRD; cb345; etID36982.3; GFAP; gfapl; wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485