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Agrisera
GI/Gigantea Polyclonal Antibody
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GI/Gigantea Antibody (AS12-1864A) in WB
50 µg of total protein from Arabidopsis thaliana extracted with TRIZOL protocol and finally dissolved in buffer E (Martínez-García et al., 1999, Plant J 20:251-7), was denatured with SDS at 95 C for 5 min, were separated on 12% (w/v) acrylamide/bis-acrylamide SDS-PAGE and blotted 10 mins to nitrocellulose using semi-dry tank transfer. Blots were blocked with 5% (w/v) skimmed milk in TBSt (Tris-Buffer Saline + 0.1% (v/v) tween-20) for 2 h at room temperature (RT) with agitation. TBSt Blot was incubated in the primary antibody at a dilution ... View More
产品信息
AS12-1864A
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
GIGANTEA is a plant circadian clock-associated gene whose product accumulates predominantly in the nucleus and functions at the interface of diurnal timekeeping and photoperiod sensing, thereby coordinating day-length-dependent developmental outputs. It encodes a large, novel protein lacking well-defined enzymatic motifs; early sequence analyses proposed multiple hydrophobic, membrane-spanning segments, but functional studies using tagged protein support a major nuclear pool consistent with transcriptional regulation of downstream targets. GI transcript abundance is under circadian control, peaking after dawn with phase and amplitude modulated by photoperiod, and loss-of-function alleles perturb clock properties (period and amplitude) as well as photoperiodic responses. Biologically, GI promotes long-day flowering by acting upstream of CONSTANS to increase CONSTANS and FLOWERING LOCUS T expression, helping align floral induction with appropriate seasonal light cycles; beyond flowering, GI contributes more broadly to circadian-regulated physiology, indicating a general role in sustaining robust rhythmic outputs across day/night conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FB; GI; GIGANTEA; T22J18.6; T22J18_6
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